期刊论文

中文

明胶科学与技术

1004-9657

2016

03

137-145+155

本校为第一机构

Collagen Assay是基于天狼星红与胶原特异性结合的原理而设计的一款新型天然胶原定量测定试剂盒。该产品已证实对于哺乳动物来源的可溶性胶原具有较好的测定效果,且方法简便。本研究尝试利用该试剂盒开展鱼类和两栖动物类胶原的定量测定,评价其测定效果及相关影响因素。测定精密度和准确度分析结果表明,该试剂盒具有较好的测定准确性、数据重现性和较强的抗杂蛋白干扰性。在此基础上,进一步开展了胶原热变性和氨基酸组成对测定结果的影响。结果表明,该试剂盒测定结果受胶原热变性程度的影响。随着胶原热变性程度的增加,其测定值逐渐减小且二者之间存在显著的线性相关性。该研究结果对于Sircol试...

Based on the principle of the combination of Sirius red and collagen,Collagen Assay reagent kit is specially designed as a new reagent kit for the quantitative determination of the soluble collagen from mammalian.In this study,the reagent kit was used for the quantitative determination of collagen in fish and amphibian,of which the measurement results and influencing factors were studied.The reagent kit's good measurement accuracy,data reproducibility and strong anti protein interference feature have been showed in this precision and accuracy analysis.Base on the analysis and studies,a further...