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Supramolecular self-assembly of high-loaded horseradish peroxidase and biorecognized antibody into Au/polydopamine nanocomposites for sensitive immunoassay of E. coli O157:H7 in milk

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成果类型:
期刊论文
作者:
Fang, Bolong;Li, Yuzhi;Zhao, Xiaole;Liu, Yan;Xu, Lin;...
通讯作者:
Liu, Xin;Gong, Zhiyong
作者机构:
[Liu, Yan; Xu, Lin; Li, Yuzhi; Wang, Qiao; Fang, Bolong; Zhao, Xiaole] Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
[Liu, Xin] Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address: liuxinhook@126.com
[Gong, Zhiyong] Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address: gongzycn@whpu.edu.cn
[Lai, Weihua] State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang 330047, China
通讯机构:
[Gong, Zhiyong; Liu, Xin] H
Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address:
语种:
英文
关键词:
Au/polydopamine/HRP;E. coli O157:H7;ELISA;enzyme loading
期刊:
Journal of Dairy Science
ISSN:
0022-0302
年:
2025
机构署名:
本校为第一且通讯机构
院系归属:
食品科学与工程学院
摘要:
It is an urgent need of rapid and sensitive method for detection of Escherichia coli O157:H7 ( E. coli O157:H7), a class of hazardous foodborne pathogens in food safety. The traditional enzyme-linked immunosorbent assay (ELISA), a dominant rapid detection technic, takes disadvantages of low test sensitivity due to the insufficient enzyme loading capacity. In this study, we successfully synthesized the self-assembled Au/polydopamine (PDA)/HRP nanocomposites with the high enzyme loading on the outer surface and in the inner space. The high cataly...

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