期刊论文

Feng, Ze-meng

英文

浙江大学学报(英文版)B辑：生物医学与生物技术

1673-1581

2015

16

6

524-532

10.1631/jzus.B1400260

Project supported by the National Natural Science Foundation of China（No.31302003） the Key Program of Hunan Province Scientific and Technology Planning Project（No.2100CK2006） the Special Fund for Innovative and Entrepreneurship Talents of Changsha City（No.K1307006-21） the Key Program of Changsha Scientific and Technology Planning Project（No.K1201108-21）,China

本校为其他机构

The energy homeostasis-associated (Enho) gene encodes a secreted protein, adropin, which regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor γ, a major regulator of lipogenesis. In the present study, the porcine (Sus scrofa) homologue of the Enho gene, which was named pEnho, was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The gene sequence was submitted into the GenBank of NCBI, and the access ...

目的：通过pEnho基因的克隆,分析p Enho基因在仔猪相关组织的分布情况,增加仔猪糖脂代谢方面相关的知识,为通过调控adropin来促进仔猪的健康和生长提供理论基础。创新点：首次克隆了猪Enho基因,命名为p Enho,其基因序列提交至美国国立生物技术信息中心（NCBI）（No.GQ414763）。方法：通过实时荧光定量逆转录聚合酶链式反应（real-time RT-PCR）方法获得猪Enho基因序列,并应用BLAST、Clustal W、PHYLIP、TMHMM、Helixturnhelix等生物信息学软件对其基因序列进行分析;通过荧光定量PCR方法检测了不同日龄（出生后1、7、14和21天）猪Enho基因的组织分布（肝脏、肌肉、空肠前端、空肠后端和回肠）。...