Orientation of fibrous collagen structures plays an important role not only in the native function of various biological tissues but also in the development of next-generation tissue engineering scaffolds. However, the controlled assembly of collagen in vitro into an anisotropic structure, avoiding complex technical procedures and specialized apparatus, remains a challenge. Here, an oriented collagen matrix was fabricated at the macroscale by simple centrifugation, and the aligned topographical features of the resulting collagen matrix were revealed by scanning electron microscopy (SEM), trans...