会议论文

Breeding Mortierella alpina saltant of high ARA production with Co60 γ-ray mutagenesis

中文

2013

112-115

中国粮油学会油脂分会第二十二届学术年会暨产品展示会

中国粮油学会油脂分会第二十二届学术年会暨产品展示会论文集

2013-09-01

上海

中国粮油学会

本校为第一机构

为获得生长活力较强、高产花生四烯酸的高山被孢霉突变株,以生物量、含油率和花生四烯酸(ARA)产量为评价指标,利用C060γ射线对出发菌株M12进行诱变育种,经筛选,选育出了一株M12-2-2突变株,其发酵生物量为30.73 g/L,含油率为40.51％,ARA产量为4.75 g/L,ARA产量比出发株提高了1.65倍,并且突变株的遗传性能稳定.

　　In order to get a Mortierella alpina which is more active and has a higher production of arachidonic acid(ARA),biomass,oil content and the production of ARA were considered as the evaluation index.The original strain M12 is managed by Co60γ-ray mutagenesis.After screening,we breed a M12-2-2 saltant.Its fermentation of biomass is 30.73 g/L.Oil length is 40.51%.ARA content is 4.75 g/L,production of which is 1.65 times higher than the orig...