Cloning, expression and immunological evaluation of a short fragment from Rv3391 of Mycobacterium tuberculosis

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Zhao, Jun-wei;Sun, Zhan-qiang;Yang, Hong-guo;Zhang, Chong-zhen;Yu, Xiao-li;...

通讯作者：

Qi, Yuan-ming

作者机构：

[Gao, Yan-feng; Qi, Yuan-ming; Zhao, Jun-wei] Zhengzhou Univ, Dept Bioengn, Zhengzhou 450001, Henan, Peoples R China.

[Zhang, Shu-lin; Guo, Xiao-kui; Zhao, Jun-wei; Sun, Zhan-qiang] Shanghai Jiao Tong Univ, Dept Microbiol & Parasitol, Sch Med, Shanghai 200025, Peoples R China.

[Yang, Hong-guo] Linyi Chest Hosp, Linyi, Shandong, Peoples R China.

[Zhang, Chong-zhen] Fudan Univ, Inst Genet, Shanghai 200433, Peoples R China.

[Wen, Zi-lu; Yu, Xiao-li] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan, Hubei, Peoples R China.

通讯机构：

[Qi, Yuan-ming] Z

Zhengzhou Univ, Dept Bioengn, Zhengzhou 450001, Henan, Peoples R China.

语种：

英文

关键词：

Rv3391;Mycobacterium tuberculosis;Serodiagnosis;Antigen;Gene expression;Circular dichroism

期刊：

ANNALS OF MICROBIOLOGY

ISSN：

1590-4261

年：

2011

卷：

期：

页码：

345-353

DOI：

10.1007/s13213-010-0148-7

基金类别：

Acknowledgments This study was supported by China MegaProjects of Science Research for the 11th Five Year Plan (No. 2008ZX10003-003-03) and Shanghai Commission of Science and Technology, PR China (No. 08410703800).

机构署名：

本校为其他机构

院系归属：

生命科学与技术学院

摘要：

Tuberculosis (TB) is still one of the greatest health care problems in the world. In order to identify antigens that may be used in the serodiagnosis of active tuberculosis, a short fragment from Rv3391 (fRv3391) of Mycobacterium tuberculosis was cloned and expressed. Its molecular weight and secondary structure elements were identified by mass spectrometry and circular dichroism. And its immunological nature was also evaluated by enzyme-linked immunosorbent assay (ELISA). The fRv3391 was expressed in Escherichia coli BL21 (DE3) with the molecu...

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Cloning, expression and immunological evaluation of a short fragment from Rv3391 of Mycobacterium tuberculosis

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