Physiological and metabolic analysis of nitrate reduction on poly-gamma-glutamic acid synthesis in Bacillus licheniformis WX-02

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成果类型：

期刊论文

作者：

Li, Xin*;Gou, Xiangyong;Long, Dan;Ji, Zhixia;Hu, Lifang;...

通讯作者：

Li, Xin

作者机构：

[Gou, Xiangyong; Hu, Lifang; Chen, Shouwen; Li, Xin; Long, Dan; Ji, Zhixia] Huazhong Agr Univ, State Key Lab Agr Microbiol, Coll Life Sci & Technol, Wuhan 430070, Peoples R China.

[Li, Xin] Hubei Univ Technol, Inst Biol Engn, Wuhan 430068, Peoples R China.

[Xu, Dihong] Huazhong Agr Univ, Inst Technol, Wuhan 430070, Peoples R China.

[Liu, Jun] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

[Chen, Shouwen] Hubei Univ, Coll Life Sci, Hubei Collaborat Innovat Ctr Green Transformat Bi, Wuhan 430062, Peoples R China.

通讯机构：

[Li, Xin] H

Huazhong Agr Univ, State Key Lab Agr Microbiol, Coll Life Sci & Technol, Wuhan 430070, Peoples R China.

语种：

英文

关键词：

Bacillus licheniformis;Poly-gamma-glutamic acid;Nitrate reduction;Overflow metabolism

期刊：

Archives of Microbiology

ISSN：

0302-8933

年：

2014

卷：

196

期：

页码：

791-799

DOI：

10.1007/s00203-014-1014-y

基金类别：

Acknowledgments This work was kindly supported by the National Natural Science Foundation of China (Grant No.31170046), Key Science & Technology Specific Projects in Wuhan and the State Key Laboratory of Agricultural Microbiology in Wuhan.

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本校为其他机构

院系归属：

生命科学与技术学院

摘要：

Nitrate is an important nitrogen source for organism, but whether and how nitrate improves poly-γ-glutamic acid (γ-PGA) production of bacterial is not clear. The effect of nitrate on γ-PGA production of Bacillus licheniformis WX-02 was investigated. By addition of 50mmol/L nitrate, the γ-PGA yield reached 12.3±0.21g/L, which increased 2.3-fold compared to the control. The mechanism of enhanced γ-PGA production was further investigated by analysis of nitrate reduction, physiology, pyruvate overflow metabolism and energy synthesis. Nitrate ...

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Physiological and metabolic analysis of nitrate reduction on poly-gamma-glutamic acid synthesis in Bacillus licheniformis WX-02

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