期刊论文

Anti-endotoxin Effects of Baicalin Extracted from Radix Scutellariae

中文

医药导报

1004-0781

2006

25

12

1237-1240

10.3870/j.issn.1004-0781.2006.12.001

国家自然科学基会资助项目（基会编号：39870872） 卫生部科学研究基金资助项目（基金编号：98-2-110）

本校为其他机构

目的 探讨黄芩中黄芩苷的抗内毒素作用.方法 将黄芩苷配成0.5%水溶液,鲎实验法进行抗内毒素定量测定;内毒素致家兔发热实验检测黄芩苷体内抗内毒素作用;脂多糖致小鼠死亡实验测定黄芩苷保护作用及黄芩苷对脂多糖致小鼠过度释放肿瘤坏死因子(TNF)和一氧化氮(NO)的影响实验,研究黄芩苷的抗内毒素作用.结果 0.833 g·mL-1黄芩苷可使4 EU内毒素降解为0.546 EU,破坏率为87.45%;0.5%黄芩苷溶液可显著降低内毒素引起的家兔体温升高,同等剂量脂多糖引起的小鼠死亡率从70.0%降为10.0%;黄芩中的黄芩苷可抑制脂多糖致小鼠血清中TNF和NO的过度释放,其抑制率呈剂量依赖性.结论 从黄芩中提取分离出的黄芩苷有抗...

Objective To probe into the anti-endotoxin effects of baicalin isolated from Radix Scutellariae. Methods A 0.5% aqueous solution of baicalin was prepared which was extracted and isolated from Radix Scutellariae, The in vitro antiendotoxin effect of baicalin was quantitatively determined with the limulus test after a mixture of endotoxin and baicalin had been incubated in a 37 ~C water bath for 1 h. The in vitro inhibitory of baicalin on the endotoxin-induced fever was measured in rabbits. The LPS-induced deaths in mice pretreated and not pretre...