期刊论文

Screening of Probiotics Inhibiting E.coli K88 in vitro

中文

中国畜牧兽医

1671-7236

2016

43

5

1355-1360

10.16431/j.cnki.1671-7236.2016.05.035

国家自然科学基金青年科学基金项目（31302089） 湖北省自然科学基金项目（2012FFBO4804）

本校为第一机构

本研究以从断奶仔猪肠道分离的益生菌为试验菌株,将大肠杆菌K88和益生菌接种到体外培养的猪小肠上皮细胞(intestinal porcine epithelial cell-1,IPEC-1)中,测定培养2.5h上清液中的乳酸脱氢酶(lactate dehydrogenase,LDH)活性,同时将益生菌和大肠杆菌K88体外混合培养2.5h,进行平板计数,统计大肠杆菌K88菌数的变化,筛选出可以抑制大肠杆菌K88的益生菌.试验结果显示,干酪乳杆菌和凝结芽孢杆菌能显著降低IPEC-1培养上清液中的LDH活性(P＜0.05),降低大肠杆菌K88对细胞的损伤;凝结芽孢杆菌能降低DMEM培养基中大肠杆菌K88的生长速度,结合模拟制粒过程及胃肠道环境的耐高温、耐酸及耐胆盐研究进行综合分...

The probiotics tested in the experiment were isolated from the intestinal of weaning piglets.The isolated probiotics and E.coli K88 were inoculated into the culture of intestinal porcine epithelial cell-1 （IPEC-1）.The activity of lactate dehydrogenase （LDH） in the supernatant was measured after incubating for 2.5 h.At the same time,the probiotics and E.coli K88 were cocultured in vitro,the number of E.coli K88 was counted and the probiotics which could be resistant to the E.coli K88 were selected 2.5 h later.The results showed that the Lact...