期刊论文

Effects of Recombinant E.coli Expressing Heat-stable Enterotoxin on Intestinal Morphology and Antioxidant Capacity in Seven Days old Piglets

中文

中国畜牧兽医

1671-7236

2017

44

9

2816-2821

10.16431/j.cnki.1671-7236.2017.09.038

国家自然科学基金项目（31302089） 湖北省自然科学基金项目（2012FFBO4804）

本校为第一机构

试验旨在研究表达耐热肠毒素（STa）的重组大肠杆菌对7日龄仔猪肠道形态结构及抗氧化功能的影响。选取24头7日龄仔猪,随机分在4个日粮处理组,分别为对照组（人工乳）,STa组（人工乳＋2×10^9 CFU重组菌LMG194-pBAD-STa）,LMG194组（人工乳＋2×10^9 CFU大肠杆菌LMG194）,K88组（人工乳＋2×10^9 CFU大肠杆菌K88）。试验第5天进行攻毒,第7天屠宰取样,测量小肠黏膜的绒毛高度和隐窝深度,检测空肠、回肠、结肠及血清中的过氧化氢酶（CAT）、总一氧化氮合成酶（TNOS）与诱导型一氧化氮合酶（iNOS）的活力及血清中丙二醛（MDA）与过氧化氢（H_2O_2）的含量。试验结果表明,与对照组相比,STa组仔猪各肠...

This experiment was conducted to investigate the effects of recombinant E.coli expressing heat-stable enterotoxin(STa) on intestinal absorption and barrier function,intestinal morphology and antioxidant capacity of 7 days old piglets.Twenty-four 7 days old piglets were allotted to four treatments:control group (artificial milk),STa group (artificial milk +-2) 109 CFU E.coli LMG194-pBAD-STa),LMG194 group (artificial milk +2 × 109 CFU E.coli LMG194),and K88 group (artificial milk +2× 109CFU E.coli K88).The pigs were treated with E.coli on the 5...