期刊论文

中文

中国畜牧兽医

1671-7236

2018

45

6

1715-1721

10.16431/j.cnki.1671-7236.2018.06.037

国家重点研发计划专项（2016YFD0501210） 国家自然科学基金项目（31572416） 武汉轻工大学科研项目（2016J08）

本校为第一机构

试验旨在建立猪流行性腹泻病毒（PEDV）感染幼龄仔猪肠道损伤模型。试验选用16头7日龄健康幼龄仔猪（杜×长×大）,随机分为两个处理组:对照组和PEDV组,每个处理8个重复,每个重复1头猪。试验期为10d,试验期间两个处理组饲喂相同的基础日粮。于试验第7天晚上对PEDV组仔猪口腔灌服PEDV病毒（剂量为104.5 TCID50）,对照组灌服等量的生理盐水。于试验第10天早上空腹灌服D-木糖（0.1g/kg体重）,1h后前腔静脉采血,屠宰取样,取十二指肠、空肠、回肠等组织样品,测定平均日增重（ADG）、腹泻率（DR）、肠道形态结构、肠道黏膜损伤相关基因mRNA水平。试验结果表明:（1）PEDV感染显著降低了仔猪ADG（P

This study was conducted to establish the intestinal injury model of porcine epidemic diarrhea virus（PEDV）infection in neonatal piglets.Sixteen crossbred healthy piglets（Ducoc×Landrace×Yorkshire,7-day-old neonatal pigs）were randomly allocated to control and PEDV groups.During a 10-day trial,all the pigs were fed the basal diet.On the 7 th day,the infected pigs were orally administrated with 104.5 TCID50 of PEDV per pig,while the control pigs were given equal amount of sterile saline.On the 10 th day,all pigs were orally gavaged with D-xyl...