期刊论文

Effects of Recombinant E.coli Expressing Heat-stable Enterotoxin on Small Intestinal Function in 7 Days Old Piglets

中文

中国畜牧兽医

1671-7236

2019

46

2

424-432

10.16431/j.cnki.1671-7236.2019.02.012

国家重大研发计划项目(2017YFD0500505) 湖北省技术创新专项(2017AHB062).

本校为第一机构

试验旨在研究表达Ⅰ型耐热肠毒素(S T a)的重组大肠杆菌对7日龄仔猪小肠功能的影响.选取24头7日龄仔猪,随机分为4个处理组,分别为对照组、STa组、LMG194组和K88组.整个试验期间均以人工乳饲喂,预饲期4 d,试验第5天进行攻毒,STa组、LMG194组及K88组分别在早晚两次口服灌喂2×109 CFU大肠杆菌LMG194-pBAD-STa、LMG194和K88,对照组灌服等量生理盐水,试验第7天早上各组灌服D-木糖并采血,屠宰并取空肠、回肠组织样品,测量仔猪血常规指标、血浆D-木糖含量及二胺氧化酶(DAO)活力,以及吸收与转运相关基因与蛋白表达量.结果显示,与对照组相比,STa组仔猪血液中中性粒细胞、中性粒细胞比率显著降低(P

This experiment was aimed to investigate the effects of recombinant E.coli expressing heat-stable enterotoxin(STa)on small intestinal function in 7 days old piglets.Twenty-four 7 days old piglets were randomly divided into four treatments of control,STa,LMG194 and K88 groups,which were fed with artificial milk throughout the experiment.Pre-feeding period was4 d.STa,LMG194 and K88 groups were orally administered with 2×109 CFU E.coli LMG194-pBAD-STa,LMG194 and K88 twice in the morning and evening on the 5 th day,respectively.The control group w...