期刊论文

中文

中国畜牧兽医

1671-7236

2019

46

4

1151-1157

10.16431/j.cnki.1671-7236.2019.04.023

2017年国家重大研究与开发项目(2017YFD0500505) 湖北省科学技术创新项目(2017AHB062).

本校为第一机构

本试验旨在研究表达Ⅰ型耐热肠毒素(STa)的重组大肠杆菌诱发7日龄仔猪小肠炎症反应.选取24头7日龄健康仔猪,随机分为4个处理组:对照组(人工乳)、STa组(人工乳+2×109 CFU重组菌LMG194-pBAD-STa)、LMG194组(人工乳+2×109 CFU大肠杆菌LMG194)和K88组(人工乳+2×109 CFU大肠杆菌K88),每组6头.试验第5天进行攻毒,第7天屠宰取样,观察空肠组织形态学变化,并检测血清中炎性细胞因子含量及空肠免疫相关基因的相对表达量.结果显示,与对照组相比,S T a与K88组仔猪空肠绒毛明显萎缩变短,有明显损伤甚至脱落;STa、LMG194及K88组血清中IL-10、IL-8和IL-6浓度均显著升高(P

This experiment was conducted to investigate the effects of recombinant E.coli expressing heat-stable enterotoxin(STa)on jejunum inflammation of 7 days old piglets.Twenty-four 7 days old piglets were allotted to four treatments:Control group(artificial milk),STa group(artificial milk+2×10^9 CFU E.coli LMG194-pBAD-STa),LMG194 group(artificial milk+2×10^9 CFU E.coli LMG194),and K88 group(artificial milk+2×10^9 CFU E.coli K88),six piglets each group.The pigs were treated with E.coli on the 5th day and slaughtered on the 7th day.The results show...