期刊论文

中文

中国畜牧兽医

1671-7236

2019

46

11

3190-3197

10.16431/j.cnki.1671-7236.2019.11.007

国家自然科学基金(31672607)。

本校为第一机构

本研究利用脂多糖(LPS)诱导建立仔猪免疫损伤模型,研究黄芩苷对LPS诱导的仔猪血管紧密连接蛋白表达量和M LCK-M LC信号通路变化的影响,并探讨其保护机制.试验选取42头健康仔猪,分为空白对照组(A组)、LPS模型组(B组)、LPS+丙酮酸乙酯处理组(C组)、LPS+氟尼辛葡甲胺处理组(D)和LPS+黄芩苷处理组(剂量分别为25、50和100 mg/kg,E、F、G组).饲喂7d后,攻毒前0.5h进行药物处理,处理组及模型组腹腔注射LPS,空白对照组注射等量生理盐水,LPS诱导6h后再次给药.试验1d后,采集主动脉血管样品2份,通过免疫组化及免疫荧光观察血管中紧密连接蛋白(闭锁小带蛋白-1 (ZO-1)、密封蛋白-1(Claudin-1)和闭合蛋白(Occlu...

In this study,lipopolysaccharide(LPS)induced immune injury model of piglets was established to study the effect of baicalin on the expression levels of LPS-induced vascular tight coupling protein in piglets and the changes of MLCK-MLC signaling pathway,and to explore its protective mechanism.Forty-two healthy piglets were selected and divided into six groups:Control group(group A),LPS model group(group B),LPS+ethyl pyruvate treatment group(group C),LPS+flunixin meglumine treatment group(group D),LPS+baicalin treatment group(25,50,100 mg/kg,groups E,F,G).After feeding for 7 d,drug treatment was...