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Abortive Infection of Snakehead Fish Vesiculovirus in ZF4 Cells Was Associated with the RLRs Pathway Activation by Viral Replicative Intermediates

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成果类型:
期刊论文
作者:
Wang, Wenwen;Asim, Muhammad;Yi, Lizhu;Hegazy, Abeer M.;Hu, Xianqin;...
通讯作者:
Lin, Li
作者机构:
[Hu, Xianqin; Asim, Muhammad; Wang, Wenwen; Yi, Lizhu; Zhou, Yang; Hegazy, Abeer M.; Lin, Li] Huazhong Agr Univ, Dept Aquat Anim Med, Coll Fisheries, Wuhan 430070, Peoples R China.
[Wang, Wenwen; Lin, Li] Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China.
[Hegazy, Abeer M.] NWRC, CLEQM, El Kanater 13621, Egypt.
[Hu, Xianqin] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430023, Peoples R China.
[Ai, Taoshan] Wuhan Fishery Res Inst, Wuhan 430207, Peoples R China.
通讯机构:
[Lin, Li] H
Huazhong Agr Univ, Dept Aquat Anim Med, Coll Fisheries, Wuhan 430070, Peoples R China.
语种:
英文
关键词:
alpha interferon;melanoma differentiation associated gene 5 protein;Myxovirus resistance protein;peptides and proteins;polyclonal antibody;retinoic acid inducible protein I;RIG I like receptor;unclassified drug;virus glycoprotein;virus nucleoprotein;DEAD box protein;MDA5 protein,zebrafish;RIG-I protein, zebrafish;zebrafish protein;animal cell;Article;cell line;controlled study;DNA synthesis;DrLGP2a gene;DrLGP2b gene;DrLGP2T gene;embryo;gene;gene amplification;gene expression;genetic transfection;nonhuman;nucleotide sequence;phylogenetic tree;polyadenylation;protein expression;real time polymerase chain reaction;RNA isolation;sequence analysis;snakehead (fish);upregulation;vesicular stomatitis;virus replication;Western blotting;ZF4 cell line;animal;genetics;metabolism;pathogenicity;physiology;signal transduction;Vesiculovirus;zebra fish;Danio rerio;RNA viruses;Vesiculovirus;Animals;Cell Line;DEAD-box RNA Helicases;Signal Transduction;Vesiculovirus;Zebrafish;Zebrafish Proteins
期刊:
International Journal of Molecular Sciences
ISSN:
1422-0067
年:
2015
卷:
16
期:
3
页码:
6235-6250
基金类别:
Huazhong Agricultural University Scientific & Technological Self-innovation Foundation [52204-12020]; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2013PY069, 2014PY035]; Wuhan Chopper Fishery Co., Ltd. [720107-137076]; Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31372563, 31402341]; Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2014M550400]; State Key Laboratory of Freshwater Ecology and Biotechnology Open Foundation [2015FB05]
机构署名:
本校为其他机构
院系归属:
动物科学与营养工程学院
摘要:
Snakehead fish vesiculovirus (SHVV) is a negative strand RNA virus which can cause great economic losses in fish culture. To facilitate the study of SHVV-host interactions, the susceptibility of zebrafish embryonic fibroblast cell line (ZF4) to the SHVV was investigated in this report. The results showed that high amount of viral mRNAs and cRNAs were detected at the 3 h post-infection. However, the expressions of the viral mRNAs and cRNA were decreased dramatically after 6 h post-infection. In addition, the expressions of interferon (IFN) and i...

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