This study was financially supported by the Open Project of Hubei Key Laboratory of Animal Nutrition and Feed Science (Grant Number 201809).
机构署名：
本校为第一且通讯机构
院系归属：
生命科学与技术学院
动物科学与营养工程学院
摘要：
In this work, the high-level expression of the human lysozyme (HLY) was achieved by both optimization of the gene copy number and co-expression of the transcription factor Hac1p for the unfolded protein response (UPR) in the host strain Pichia pastoris KM71H. A series of recombinant constructs with various numbers of HLY expression cassettes was generated for the production of recombinant strains integrated with different copies of the HLY gene. The copy number of the HLY gene was determined by real-time quantitative polymerase chain reaction, ...
