Hubei Provincial Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [2008CDA100]; teacher scientific innovation program of Wuhan Polytechnic University [2012Y27]
机构署名:
本校为第一且通讯机构
院系归属:
食品科学与工程学院
化学与环境工程学院
摘要:
A simple and rapid spectrophotometric method is described for the determination of trace zinc(II) in zinc-enriched yeast using 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (5-Br-PADAP) as a chromogenic reagent in the presence of surfactant OP. The analytical parameters such pH, reagent and coexistent ions were investigated. The results showed that the maximum absorption wavelength of the complex Zn-5-Br-PADAP was identified at 556 nm. The apparent molar absorptivity was 1.16 x 10(5) L mol(-1) cm(-1). Beer's law was obeyed in the concentration range of 0 similar to 12 mu g/25 mL for zinc. The ...