The culture medium and fermentation conditions were investig ated for improving the production of antagonistic substances by Actinomycetes 327# in shaking flasks. By single factor and orthogonal experiment, the optimal fermentation medium of 327# were obtained as follows： soybean flour 0.5%, （NFL）2SO4 1.5%, glucose 2.5%, NaC1 0.6%, CaCO3 0.6%. The optimal fermentation conditions were as follows： culture temperature 30~C, initial pH 7.0, culture volume 15 ml in 250 mL shaking flask. Under these conditions, the inhibition zone diameter of 327...
