To obtain the optimal fermentation condition of Crypthecodinium cohnii,single factor test was used to investigate the effect of inoculum,culture time,culture temperature,liquid volume,and medium initial pH on DHA yield.On the basis of single factor test,factors ofinoculum,medium initial pH,and liquid volume were selected as the main influence factors.Through the Box-Behnken design,the optimal fermentation conditions were acquired as follows：inoculum 9.2％,initial pH 6.9,liquid volume 65 ml/500 ml.Under such condition,the yield of ...
