期刊论文

中文

中国酿造

0254-5071

2015

34

4

106-109

10.11882/j.issn.0254-5071.2015.04.024

基金项目：国家粮食局粮食公益性行业科研专项（201313012-03）

本校为第一机构

利用60Co-γ射线辐照诱变裂壶藻(Schizochytrium limacinum) ATCC20888,以平板生长情况、生物量、含油率和二十二碳六稀酸(DHA)产量为综合指标,最终选育出一株高产突变株,命名为1.6-7-1,其摇瓶发酵生物量为47.37 g/L,油脂含量为31.41％,DHA产量为5.65 g/L,且DHA产量较出发菌株提高了约40％,经连续5代发酵培养,其遗传性能稳定.

Schizochytrium limacinum ATCC20888 was mutated by 60Co-γ ray irradiation. Using flat growth condition, biomass, oil content and docosahexaenoic acid （DHA） yield as comprehensive indexes, a mutant strain 1.6-7-1 with high DHA yield was screened. The biomass of the strain was 47.37 g/L, oil content was 31.41%, and DHA yield was 5.65 g/L, which was increased by about 40% than the original strain. After five consecutive generations o...