期刊论文

Determination of 29 pesticides residues in vegetables by QuEChERS-HPLC-MS/MS

中文

中国酿造

0254-5071

2019

38

09

190-195

10.11882/j.issn.0254-5071.2019.09.037

湖北省自然科学基金计划项目(No.2018CFB340)资助；

本校为第一机构

建立了QuEChERS-高效液相色谱-串联质谱(HPLC-MS/MS)同时快速测定蔬菜中29种农药残留的分析方法。样品以乙腈提取,氯化钠盐析,以十八烷基硅烷键合硅胶(C18)和N-丙基乙二胺(PSA)净化,以WatersXBridgeC18色谱柱分离,电喷雾正离子扫描,多反应监测(MRM)模式检测,基质匹配标准溶液外标法定量。在此优化条件下,29种农药分子在不同加标水平下的平均回收率为64.62%~123.55%,相对标准偏差(RSD)为0.7%~14.3%,方法定量限(LOQ)为0.40~8.00μg/kg。该方法简便快速、灵敏度高、重现性好,能满足蔬菜中农药残留的快速检测要求。

A rapid analytical method for simultaneous determination of 29 pesticides residues in vegetables was developed using QuEChERS-HPLC- MS/MS.The samples were extracted with acetonitrile,salted out with sodium chloride,and then the extract was cleaned up with ostadecylsilane (C18) and primary secondary amine (PSA).The target compounds were separated on a Waters XBridge C18 chromatographic column,determined using electrospray ionization (ESI) source in positive mode with the multiple reaction monitoring (MRM) mode and quantified with the matrix-matc...