The paper studied ferment condition of Claviceps paspali to producing ergot alkali. The main factors which affected ergot alkali production were tryptophane and succinamide by the method of latin square L18(37). Single factor test was further used to analyzing the rule of ergot alkali production. The ideal incubation condition was determined as following: tryptophane 0. 1%, mannitol 10%, succinamide 4%, MgSO4·7H2O 0. 025%, pH 5. 2 adjusted with ammonia, 24-26℃ in shake flasks for 11-13 d. The average conten...