期刊论文

Strain Improvement of Lysergic Acid Amid Producing Claviceps Paspali by Protoplast Mutagenesis

中文

化学与生物工程

1672-5425

2005

022

7

33-35,45

10.3969/j.issn.1672-5425.2005.07.012

湖北省教育厅科研资助项目（2002A01009）；

本校为第一机构

为了提高在一般条件下不易产生分生孢子的雀稗麦角菌的产碱率,对原生质体诱变育种的条件和方法进行了探讨.实验表明,对麦角酰胺产生菌的原生质体用紫外线照射5 min、7 min、9 min进行诱变处理,可获得较稳定的高产突变株.麦角酰胺的产率由原始菌株的58 mg·L-1提高到459 mg·L,增加了约7倍,连续传代培养可以得到稳定的高产菌株,说明用原生质体进行诱变处理,对提高麦角酰胺的产率是可行的.

The feasibility of the protoplast mutagenesis for increasing productivity was studied on a Claviceps paspali strain, which was not easy to produce conidia under laboratory conditions. The experiments showed that the stable mutants with increased titers were obtained by UV-irradiation for five minutes, seven minutes and nine minutes, respectively. After UV-irradiation the lysergic acid amide alkaloid productivity was increased by 8 times, from the initial titers 58 mgL~(-1) to 459 mgL~(-1) after continual transfers. The results indicated that the protoplast UV-irradiation was a feasible approac...