期刊论文

Optimization of Cellulase Extraction Process of Waste Material from Mushroom（Lentinus edodes）

中文

江西农业学报

1001-8581

2016

28

5

83-87

10.3969/j.issn.1001-8581.2016.05.018

武汉轻工大学引进(培养)人才科研启动项目(2013RZ04)； 国家科技支撑计划项目(2012BAD36B05)；

本校为第一机构

以香菇菌糠为原料,采用超声浸提法制备纤维素粗酶液,通过单因素试验和响应面设计,分析了超声功率、超声时间和液料比等因素对纤维素酶浸提的影响。结果表明,香菇菌糠纤维素酶最佳提取工艺为：超声功率500 W,超声时间15 min,液料比为20∶1,在此条件下的CMC酶活力为9.61 IU/g。

Cellulase of the waste material from Lentinus edodes was prepared by ultrasonic extraction, the effects of ultrasonic power, ultrasonic time and liquid-solid ratio on the activity of cellulase were studied through single factor tests and response sur-face analysis.The results showed that the optimum process parameters were obtained as follows:ultrasonic power was 500 W, ul-trasonic time was 15 min, liquid ratio was 20∶1, three validation tests under these condition...