期刊论文

中文

中国生物工程杂志

1671-8135

2016

36

2

43-50

10.13523/j.cb.20160207

湖北省支撑计划项目

本校为第一机构

细菌血红蛋白可增加细胞在低氧条件下的氧气利用率,提高细胞的增长速率。为提高E. coli在现有发酵水平下的生物量,获得新型的基因工程底盘细胞,基于空肠弯曲杆菌(Campylobacter jejuni)血红蛋白基因,比较分析了组成型和诱导型启动子对血红蛋白在E. coli基因工程菌中的表达状况及对E. coli生长的影响。首先合成了空肠弯曲杆菌血红蛋白基因chb,并让chb基因在诱导型启动子(PT7和Pvgh)和组成型启动子(P2和PspoI-II)控制下表达;随后,测定了由四种类型启动子控制的血红蛋白工程菌在摇瓶和蓝盖瓶中的生长状况。结果表明四种重组菌可以表达出有活性的血红蛋白,并可显著提高大肠杆菌的生长;在发酵罐中的试...

Bacteria hemoglobin could improve the cell＇ s oxygen utility and enhance the cell growth rate under low oxygen environment. To improve the biomass of E. coli and supply a novel type of host cell for bioengineering, the effect of four types of promoter controlled Campylobacter jejuni hemoglobin on the growth of E. coli cells was comparatively analyzed. The C. jejuni hemoglobin chb was initially synthesized and then it was expressed under the control of inducible promoter PT7 and Pvsh, constitutive promoter P2 and Pspoi-li- The growth curves of ...