期刊论文

Improving the Activity and Thermal Stability of Thermomyces lanuginosus Lipase by Rational Design

中文

中国生物工程杂志

1671-8135

2021

41

Z1

63-69

10.13523/j.cb.2011036

本校为第一机构

目的:通过对疏棉状嗜热丝孢菌（Thermomyces lanuginosus）脂肪酶的理性设计,获得高酶活与耐高温的脂肪酶品种,为脂肪酶在饲料、油脂加工和生物柴油等领域的应用奠定基础。方法:对脂肪酶典型结构域lid和loop区域的系统发育分析,找到候选的位点,理性设计并通过实验验证,获得脂肪酶活性和耐高温特性显著提高的脂肪酶重组子,并构建多拷贝载体,完成50L发酵罐中进行产酶能力评价。结果:经过设计的脂肪酶高温稳定性显著提升。其在80℃下放置12h后仍保留78.94%的酶活。脂肪酶重组子在50L发酵罐中发酵诱导168h,其上清液酶活达到29 000U/m L。结论:成功设计并获得了一种新型的高活性、耐高温的脂肪酶品种...

Objective: In order to obtain a lipase with high enzyme activity and high temperature resistance, the Thermomyces lanuginosus lipase was modified by rational design,which laid the foundation for the application of lipase in the feed industry,oil processing and biodiesel. Methods: A candidate site was found by phylogenetic analysis of the lid and loop regions of the typical domain of lipase. A lipase recombinant with significantly improved lipase activity and high temperature resistance was obtained through rational design and experimental verif...