期刊论文

中文

生物工程学报

1000-3061

2023

39

11

4635-4646

10.13345/j.cjb.230224

中国博士后科学基金(2019M661032) 天津市合成生物技术创新能力提升行动(TSBICIP-CYFH-011)。

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灯盏乙素发酵生产过程中,黄酮6位羟基化酶催化效率不足,导致产生至少约18%的副产物。本研究以2种黄酮6位羟基化酶CYP82D4与CYP706X为研究目标,通过分子动力学模拟与量子化学计算,对两种黄酮6位羟基化酶的催化机制...展开更多 灯盏乙素发酵生产过程中,黄酮6位羟基化酶催化效率不足,导致产生至少约18%的副产物。本研究以2种黄酮6位羟基化酶CYP82D4与CYP706X为研究目标,通过分子动力学模拟与量子化学计算,对两种黄酮6位羟基化酶的催化机制进行解析。结果表明,CYP82D4与CYP706X在反应决速步的能垒几乎相同,应当具有相似的反应速率,而CYP82D4相对较小的底物结合能可能有利于产物释放,是其具有更高催化...

Insufficient catalytic efficiency of flavonoid 6-hydroxylases in the fermentative production of scutellarin leads to the formation of at least about 18%of by-products.Here,the catalytic mechanisms of two flavonoid 6-hydroxylases,CYP82D4 and CYP706X,were investigated by ...MORE Insufficient catalytic efficiency of flavonoid 6-hydroxylases in the fermentative production of scutellarin leads to the formation of at least about 18%of by-products.Here,the catalytic mechanisms of two flavonoid 6-hydroxylases,CYP82D4 and CYP706X,were investigated by molecular dynamics simulations and quantum chemical ...