Rapid Detection of Single Viable Escherichia coli O157 Cells in Fresh Lettuce and Strawberry by Immunomagnetic Flow Cytometry in Combination with Pre-Enrichment

首页 > 成果 > 详情

认领

导出

Link by DOI

反馈

作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Wang, Ziquan;Liu, Siyuan;Zhen, Xiaoxiao;Li, Longquan;Zhou, Guoping;...

通讯作者：

Sui, Z.;Wei, Q.

作者机构：

[Wang, Ziquan; Liu, Siyuan; Sui, Zhiwei] Center for Advanced Measurement Science, National Institute of Metrology, No.18, Bei San Huan Dong Lu, Chaoyang District, Beijing, 100029, China

[Zhen, Xiaoxiao] Institute of Chemical Analysis and Biomedicine, Beijing Institute of Metrology, Beijing, China

[Zhou, Guoping] School of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China

[Wei, Qiang] National Pathogen Resource Center, Chinese Center for Disease Control and Prevention, 155 Changbai Road Changping District, Beijing, 102206, China

[Li, Longquan] Center for Advanced Measurement Science, National Institute of Metrology, No.18, Bei San Huan Dong Lu, Chaoyang District, Beijing, 100029, China, School of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China

通讯机构：

[Qiang Wei] N

[Zhiwei Sui] C

Center for Advanced Measurement Science, National Institute of Metrology, Beijing, China.<&wdkj&>National Pathogen Resource Center, Chinese Center for Disease Control and Prevention, Beijing, China.

语种：

英文

关键词：

Escherichia coli O157;flow cytometry;immunomagnetic-beads;lettuce;single cell;strawberry

期刊：

FOODBORNE PATHOGENS AND DISEASE

ISSN：

1535-3141

年：

2022

卷：

期：

页码：

36-44

DOI：

10.1089/fpd.2021.0031

基金类别：

This study was supported by the National Key Research and Development Program of China (No. 2017YFF0204602), the National Key Research and Development Program of China (No. 2018ZX10734404), the Science and Technology Program of the State Administration for Market Regulation (No. 2019MK114), and the Fundamental Research Funds for Central Public Welfare Scientific Research Institutes sponsored by the National Institute of Metrology, P.R. China (Nos. AKY1818 and AKY1958).

机构署名：

本校为第一机构

院系归属：

生命科学与技术学院

摘要：

Enterohemorrhagic Escherichia coli are an important pathogen causing food poisoning. The rapid detection of viable E. coli O157 in vegetables and fruits at single-cell level is critical because of the low infective dose of this pathogen. In this study, an immunomagnetic flow cytometry (IMFC)-based method was developed to detect E. coli O157 in lettuce and strawberries inoculated with 1 CFU/25 g. This method developed immunomagnetic (IM)-beads to capture E. coli O157 cells. The pre-enrichment of E. coli O157 and IM-bead separation rapidly increa...

反馈

产权有误：本人成果被他人认领

数据有误：数据基本信息有误

归属有误：成果的院系归属、机构署名归属有误

其他原因：

验证码：

看不清楚，换一个

确定

取消

成果认领

标题：

用户	作者	通讯作者	--
	请选择	请选择	--

确定

取消

Rapid Detection of Single Viable Escherichia coli O157 Cells in Fresh Lettuce and Strawberry by Immunomagnetic Flow Cytometry in Combination with Pre-Enrichment

反馈

成果认领

提示

该栏目需要登录且有访问权限才可以访问