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Analysis of microRNA expression profiles in porcine PBMCs after LPS stimulation

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成果类型:
期刊论文
作者:
Zhang, Jing;Xu, Xin;Huang, Xingfa;Zhu, Huiling;Chen, Hongbo;...
通讯作者:
Liu, Yulan;Wang, Wenjun
作者机构:
[Zhang, Jing; Liu, Yulan; Xu, Xin; Zhu, Huiling; Chen, Hongbo] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
[Wang, Wenjun; Huang, Xingfa] South Cent Univ Nationalities, Hubei Prov Key Lab Protect & Applicat Special Pla, Wuhan, Peoples R China.
[Wang, Wenjun] Hubei Prov Key Lab Protect & Applicat Special Pla, Wuhan 430074, Peoples R China.
通讯机构:
[Liu, Yulan] W
[Wang, Wenjun] H
Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
Hubei Prov Key Lab Protect & Applicat Special Pla, Wuhan 430074, Peoples R China.
语种:
英文
关键词:
PBMCs;LPS;microRNA;small RNA sequencing;pig
期刊:
INNATE IMMUNITY
ISSN:
1753-4259
年:
2020
卷:
26
期:
5
页码:
435-446
基金类别:
This study was supported by the National Natural Science Foundation of China (31702203), the Open Project of Hubei Key Laboratory of Animal Nutrition and Feed Science (grant number 201908), the Project of the Hubei Provincial Department of Education (T201508) and the projects of Wuhan Science and Technology Bureau (2018020401011304).
机构署名:
本校为第一且通讯机构
院系归属:
动物科学与营养工程学院
摘要:
In the present study, we used microRNA (miRNA) sequencing to discover and explore the expression profiles of known and novel miRNAs in 1000 ng/ml LPS stimulated for 8 h vis-à-vis non-stimulated (i.e. control) PBMCs isolated from the blood of healthy pigs. A total of 291 known miRNAs were bio-computationally identified in porcine PBMCs, and 228 novel miRNAs (not enlisted in the swine mirBase) were identified. Among these miRNAs, ssc-miR-148a-3p, ssc-let-7g, ssc-let-7f, 3_8760, ssc-miR-26a, ssc-miR-451, ssc-miR-21, ssc-miR-30d, ssc-miR-99a and s...

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