期刊论文

Effects of Flaxseed Oil on mRNA Expression of Key Genes in TLR4 and NOD Signaling Pathways in Liver of Piglets after LPS Challenge

中文

动物营养学报

1006-267X

2016

28

3

891-898

10.3969/j.issn.1006-267x.2016.03.031

湖北省自然科学基金***人才项目

本校为第一机构

本试验旨在研究亚麻籽油对脂多糖(LPS)刺激仔猪肝脏Toll样受体4(TLR4)和核苷酸结合寡聚化结构域(NOD)信号通路关键基因表达的影响。选取24头断奶仔猪,按体重相近原则随机分为4个组,分别为对照组、LPS组、2.5%亚麻籽油组(2.5%亚麻籽油+LPS)、5.0%亚麻籽油组(5.0%亚麻籽油+LPS),每组6个重复,每个重复1头猪,试验期21 d。试验组注射100 μg/kg体重的LPS,对照组注射等量的生理盐水。注射LPS或生理盐水4 h后屠宰仔猪,取肝脏,测定TLR4和NOD信号通路关键基因及相关炎性介质的mRNA表达水平。结果表明:1)LPS刺激显著提高了肝脏肿瘤坏死因子-α(TNF-α)、环氧酶2(COX2)、热休克蛋白70(HSP70)的mRNA相对表达...

This experiment was conducted to investigate the effects of flaxsee d oil on mRNA expression of key genes in Toll-like receptor 4 ( TLR4) and nucleotide binding oligomerization domain protein ( NOD) signaling pathways in liver of piglets after lipopolysaccharide ( LPS ) challenge.Twenty four pigs with similar body weight were randomly divided into 4 groups with 6 replicates per group and 1 pig per replicate, and the 4 groups were control group, LPS group, 2.5%flaxseed oil group (2.5%flaxseed oil +LPS), and 5.0%flax-seed oil group (5.0%flaxseed ...