期刊论文

Effects of Lipopolysaccharide Challenge on Key Genes Expression of Inflammatory Signaling Pathway in Spleen of Weaned Piglets

中文

动物营养学报

1006-267X

2019

31

8

3882-3890

10.3969/j.issn.1006?267x.2019.08.050

湖北省教育厅优秀中青年科技创新团队项目（T201508）；

本校为第一机构

本试验旨在研究脂多糖(LPS)刺激后不同时间断奶仔猪脾脏Toll样受体4( TLR4)和核苷酸结合寡聚域受体( NOD)炎症信号通路关键基因表达的变化.选择42 头体重为( 7.1 ± 0.9) kg的杜×长×大断奶仔猪,按注射LPS之前( 0 h)和注射LPS后不同时间点( 1、2、4、8、12、24 h)随机分为7个处理,每个处理6头猪.预饲14 d后,腹腔注射100 μg／kg BW的LPS,分别于注射LPS之前和注射LPS后不同时间点,将仔猪麻醉屠宰,取血液和脾脏样品,测定血细胞分类计数和脾脏炎症信号通路关键基因及炎症相关基因的mRNA表达量.结果表明:LPS刺激导致部分血细胞分类计数发生显著的变化( P＜0.05) ;使 TLR4 和 NOD 炎症信号通路关键基...

The purpose of this study was to investigate the changes of key genes of Toll?like receptor 4 ( TLR4) and nucleotide?bound oligomeric domain receptor ( NOD) inflammation signaling pathway in spleen of weaned piglets at different time after lipopolysaccharide ( LPS) challenge. Forty?two Duroc×Landrace× Yorkshine weaned piglets with a body weight of (7.1±0.9) kg were selected and randomly divided into 7 treat?ments ( 6 pigs per treatment). The piglets were injected with 100 μg／kg body weight LPS, and slaughtered at 0 h ( before LPS challenge...