期刊论文

Effects of Tumor Necrosis Factor-α Stimulation on Viability, Physical Barrier and Inflammation-Related Gene Expression of IPEC-1 Cells

中文

动物营养学报

1006-267X

2020

32

9

4337-4344

10.3969/j.issn.1006⁃267x.2020.09.044

(2019CFA015):湖北省自然科学基金创新群体项目"细胞死亡与猪肠道疾病的关系及其营养调控"

本校为第一机构

本试验探讨了肿瘤坏死因子-α( TNF-α)刺激对IPEC-1细胞活力、乳酸脱氢酶( LDH)活性、紧密连接蛋白和炎症相关基因表达的影响。分别选取0(对照组) 、10、20、40、50 ng / mL的TNF-α刺激IPEC-1细胞12、24、48 h,检测相关指标。结果表明:1)与对照组相比,当刺激时间为24 h时,不同浓度的TNF-α均导致细胞活力显著下降( P

This study was investigated the effects of tumor necrosis factor-α (TNF-α) stimulation on viability, lactate dehydrogenase ( LDH ) activity, tight junction protein and inflammation-related gene expression of IPEC-1 cells. Selected 0 (control group), 10, 20, 40 and 50 ng / mL of TNF-α to stimulate IPEC-1 cells for 12, 24 and 48 h, respectively, and detected relevant indexes. The results showed as follows: 1) compared with the control group, different concentrations of TNF-α significantly decreased cell viability at stimulation for 24 h (P