期刊论文

Effects of Lipopolysaccharide on Inflammation and Protein Degradation-Related Genes and miRNA Expression in Gastrocnemius of Piglets after 24 h Stimulation

中文

动物营养学报

1006-267X

2021

33

10

5802-5808

10.3969/j.issn.1006-267x.2021.10.039

湖北省自然科学基金创新群体项目（2019CFA015）;湖北省自然科学基金面上项目（2019CFB831）;湖北省自然科学基金面上项目（2018CFB527）；

本校为第一机构

本试验旨在探讨脂多糖(LPS)刺激24 h后,与仔猪腓肠肌炎症和蛋白质降解相关基因与miRNA的表达变化。试验选用12头健康的28日龄断奶的杜×长×大三元杂交仔猪[体重为(7.09±0.90) kg],随机分为对照组(n = 6)和LPS组(n = 6),所有试验猪自由采食和饮水,预试期为15 d。在试验第15天,LPS组仔猪腹腔注射100 μg / kg BW的LPS,并于注射24 h后麻醉屠宰,对照组仔猪则腹腔注射等量的无菌生理盐水,并于注射后立即麻醉屠宰,取腓肠肌样品待测。结果显示:LPS处理24 h后,腓肠肌炎症细胞因子白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α),以及Toll样受体4(TLR4)信号通路关键基因TLR4、骨髓分化因子88(MyD88) ...

This experiment was conducted to investigate the expression changes of genes and miRNA related to inflammation and protein degradation in gastrocnemius of weaned piglets challenged by lipopolysaccharide (LPS) at 24 h. Twelve healthy Duroc×Landrace×Yorkshine 28-day-old weaned piglets [body weight: (7.09± 0.90) kg] were selected, and randomly divided into 2 groups: control group (n = 6) and LPS group (n = 6). All piglets were allowed ad libitum access to water and feed. On day 15 of experiment, the piglets in LPS group were injected with LPS (...