Explore a method collecting endothelial progenitor cells from human umbilical cord blood, then culture and identify these cells. Endothelial progenitor cells were obtained from human umbilical cord blood by Ficoll density gradient separation, purified by magnetic cell sorting (MACS), than cultured them in cord blood. Identified these cells by flow cytometry (FCM) and fluorescent immune staining method. Result endothelial progenitor cells grew well. Endothelial progenitor cells could be obtained from human umbilical cord blood, it was good for furt...
