Program for New Century Excellent Talents in University of ChinaProgram for New Century Excellent Talents in University (NCET) [NCET-07-0341]; National High-Tech Research and Development Program of ChinaNational High Technology Research and Development Program of China [2008AA10Z317]
机构署名：
本校为其他机构
院系归属：
生命科学与技术学院
摘要：
The glr gene, which encodes glutamate racemase involved in the conversion of l-glutamic acid to its D-isomer, was cloned and expressed in Bacillus licheniformis WX-02. Overexpression of the glr gene not only increased the production of poly-γ-glutamic acid (γ-PGA) by 22.5% but also increased the proportion of d-glutamate in γ-PGA from 77 to 85%. The activity of glutamate racemase was higher than in the original strain throughout cultivation. This is the first report that overexpression of the glr gene could enhance the l- and d-glutamate con...
