期刊论文

中文

环境科学与技术

1003-6504

2022

45

2

30-36

10.19672/j.cnki.1003-6504.1885.21.338

国家自然科学基金(32070098)。

本校为第一机构

六氯丁二烯是一种新型的持久性有机污染物,利用微生物降解环境中六氯丁二烯是一种无二次污染、非常绿色高效的方法。该研究从武汉东西湖区马池桥附近污泥中采样,通过连续传代驯化,分离筛选到一株能够以六氯丁二烯为唯一碳源生长的菌株,具有高效降解六氯丁二烯(HCBD)能力。16S rRNA基因测序鉴定菌株;通过气相色谱法和气相色谱-质谱联用法来测定底物降解及产物生成情况,并对降解菌株的抗生素抗性和底物广谱性进行探究。结果表明,该菌的16S r RNA序列与根癌农杆菌(Agrobacterium tumefaciens)具有99%的一致性,构建系统发育树显示该菌株与Agrobacterium tumefaciens聚为一支,将其命名为Agrobacteriu...

In order to use microbial degradation methods to solve the pollution of hexachlorobutadiene in the environment,the highly efficient HCBD degrading bacterium was screened from the sludge near Machiqiao in Dongxihu District, Wuhan,and its degradation characteristics was studied. This study provide theoretical basis and technical support for the treatment of hexachlorobutadiene pollutants in wastewater and soil. The bacterium was screened by continuous passage culture, enrichment, and isolation. It was identified by 16S rRNA gene and physiological and biochemical assays. The growth characteristic...