期刊论文

中文

食品科技

1005-9989

2015

40

2

17-21

10.13684/j.cnki.spkj.2015.02.004

国家自然科学基金项目（31201320）；

本校为第一机构

以冷鲜猪肉为研究对象,经STAA选择性培养基培养从冷鲜猪肉中分离得到单一菌株,经鉴定为热杀索丝菌。对热杀索丝菌产酶特性进行研究,结果表明,其最佳产酶条件为温度20℃、接种浓度106 cfu/m L、摇床速度200 r/min、培养60 h时,酶活力达到最大值。经过80%（NH4）2SO4沉淀和透析的初步纯化处理后,得到热杀索丝菌粗酶液。

As the research object in this paper. we can obtain a strain of Brochothrix thermosphacta from chilled pork after cultivated in STAA medium. This strain is identified to be Brochothrix thermosphacta. The enzyme-producing specialities were studied. The results show： Under the conditions of 20 ℃, 106 cfu/m L concentration of bacterium suspension, 200 r/min cranking speed and incubation time 60 h, we can obtain the nutrient solution with the highest enzyme activity. The crude nutrient solution was deposits by（NH4）2SO4 solution concentration of 80% and...