期刊论文

中文

食品科技

1005-9989

2016

41

3

105-110

10.13684/j.cnki.spkj.2016.03.022

国家自然科学基金青年基金项目（31201351） 湖北省自然科学基金项目（2013CFB481）

本校为第一机构

目的:研究DHA/EPA比例对高脂膳食小鼠肝脏氧化应激和炎症因子表达的影响。方法:将40只雄性apo E-/-小鼠随机分为4组并给予高脂膳食,同时用葵花籽油、紫苏油、鱼油和藻油等调节膳食中n6/n3 PUFA的比例为4:1或DHA/EPA的比例（2:1、1:1和1:2）。12周后,用商业试剂盒或实时荧光定量PCR法检测小鼠血清或肝脏组织的氧化应激水平或炎症因子表达变化。结果:DHA/EPA替代植物来源的n3 PUFA-ALA并不能减少血清脂质过氧化产物MDA生成及提高血清和肝脏的SOD活性;与补充ALA相比,DHA/EPA为1:1时小鼠血清GSH含量显著升高（P

Objective：To investigate the ratios of DHA/EPA on oxidative stress and inflammatory factors in mice treated with high-fat diets.Methods：Forty male apo E-/-mice were randomly divided into 4 groups and were given high-fat diets.Meanwhile,sunflower seed,perilla,fish,and algal oils were used for adjusting the proportion of n6/n3 PUFA 4：1 and DHA/EPA（2：1,1：1,and 1：2）.After 12 weeks feeding,commercial kits and/or real-time PCR were used to test the redox status and inflammatory factors expression in mice serum and/or liver.Resuts：Replacement...