The inhibition of γ-cyclodextrin on α-galactosidase was studied in this article.The relative activity was the evaluation index,its found that the inhibition of γ-cyclodextrin on α-galactosidase was different by the single factor,and the optimum parameters of γ-cyclodextrin inhibiting α-galactosidase activity were obtained by orthogonal experiments,the γ-cyclodextrin concentration was 10 mmol/L,reaction time was 90 min,temperature was 35 ℃,p H was 6....