This thesis regard hesperidin standards as the research object,testified and compared with current analytical methods of hesperidin standards.A simple,fast quantitative detection of hesperidin was achieved by optimizing methods.The pretreatment methods:Hesperidin standards were dissolved in methanol for 10 min by using ultrasonator,then 30%ultrapure water was added to solution.Hesperidin standards were difficult to dissolve.Methanol and ultrasonic extraction were introduced,which can improve the solubility of hesperidin standards.Additional water can effectively eliminate the chromatographic f...