α-Tocopherol, γ-tocopherol and δ-tocopherol were successfully separated and purified from soybean vitamin E concentrate (53% purity, from soybean oil deodorizer distillate) using a low-pressure glass column (500 mm ×25 mm, I.D., packed with silica gel). The effects of eluent, flow-rate and sample loading on separation efficiency were investigated using product purity and recovery as evaluation indices. On the basis of the single factor experiment results, an orthogonal test was designed to optimize the chromatographic separation conditions. The optimum conditions obtain...
