Synthesis of amino-rich silica-coated magnetic nanoparticles for the efficient capture of DNA for PCR

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成果类型：

期刊论文

作者：

Bai, Yalong;Cui, Yan;Paoli, George C.;Shi, Chunlei;Wang, Dapeng;...

通讯作者：

Shi, Xianming

作者机构：

[Zhang, Lida; Zhou, Min; Shi, Xianming; Cui, Yan; Wang, Dapeng; Bai, Yalong; Shi, Chunlei] Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Shanghai 200240, Peoples R China.

[Zhang, Lida; Zhou, Min; Shi, Xianming; Cui, Yan; Wang, Dapeng; Bai, Yalong; Shi, Chunlei] Shanghai Jiao Tong Univ, Bor Luh Food Safety Ctr, Sch Agr & Biol, Shanghai 200240, Peoples R China.

[Zhang, Lida; Zhou, Min; Shi, Xianming; Cui, Yan; Wang, Dapeng; Bai, Yalong; Shi, Chunlei] Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China.

[Paoli, George C.] ARS, USDA MOST Joint Res Ctr Food Safety, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA.

[Paoli, George C.] ARS, Mol Characterizat Foodborne Pathogens Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA.

通讯机构：

[Shi, Xianming] S

Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Shanghai 200240, Peoples R China.

Shanghai Jiao Tong Univ, Bor Luh Food Safety Ctr, Sch Agr & Biol, Shanghai 200240, Peoples R China.

Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China.

语种：

英文

关键词：

Amino-rich;Bio-separation;DNA;Magnetic nanoparticles;PCR

期刊：

Colloids and Surfaces B: Biointerfaces

ISSN：

0927-7765

年：

2016

卷：

145

页码：

257-266

DOI：

10.1016/j.colsurfb.2016.05.003

基金类别：

This study was supported by the National Natural Science Foundation of China ( 31230058 and 31471660 ), Shanghai Agriculture Commission Project (2015, NO. 4-5 ), and Shanghai Jiao Tong University ( AgriX2015005 ).

机构署名：

本校为其他机构

院系归属：

食品科学与工程学院

摘要：

Magnetic separation has great advantages over traditional bio-separation methods and has become popular in the development of methods for the detection of bacterial pathogens, viruses, and transgenic crops. Functionalization of magnetic nanoparticles is a key factor for efficient capture of the target analytes. In this paper, we report the synthesis of amino-rich silica-coated magnetic nanoparticles using a one-pot method. This type of magnetic nanoparticle has a rough surface and a higher density of amino groups than the nanoparticles prepared...

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Synthesis of amino-rich silica-coated magnetic nanoparticles for the efficient capture of DNA for PCR

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