期刊论文

Construction of Genetically Engineered Bacterium of Homogentisate Dioxygenase

中文

基因组学与应用生物学

1674-568X

2019

38

6

2627-2634

10.13417/j.gab.038.002627

国家自然科学基金项目（31460027）资助；

本校为其他机构

为了构建不同表达类型的尿黑酸双加氧酶(Dio6)基因工程菌,本研究利用Over-lap PCR技术使双加氧酶基因在组成型启动子(P2和P_(spoⅠ-Ⅱ))的控制下表达;利用高效液相色谱(HPLC)技术测定XJ-6、XJ-6+P_(T7)、 XJ-6+P2、XJ-6+P_(spoⅠ-Ⅱ)、P_(spoⅠ-Ⅱ)、P2、P_(T7)在7 d对芘的降解。研究结果表明:构建得到3种启动子控制表达的基因工程菌,协同效应研究表明工程菌在XJ-6的协助下可以在含芘的无机盐培养基中生长,并且组成型启动子工程菌的菌数远高于诱导型启动子工程菌的菌数。组成型启动子相较于诱导型启动子工程菌能更稳定表达外源双加氧酶基因,由于诱导型启动子工程菌需要诱导剂IPTG,并且表达双加氧...

In order to construct different expression types of engineering bacteria that express homogentisate dioxygenase (Dio6 gene), Overlap PCR technology was used to make the expressions of dioxygenase under the control of the constitutive promoter (P2 and P_(spoⅠ-Ⅱ)), and then the degradation rates of pyrene by the strains of XJ-6, XJ-6+P_(T7), XJ-6+P2, XJ-6+P_(spoⅠ-Ⅱ), P_(spoⅠ-Ⅱ), P2 and P_(T7) were determined by HPLC. The research results showed that genetically engineered bacteria controlled by three promoters were successfully constructed ...