期刊论文

中文

粮食与油脂

1008-9578

2018

31

5

66-70

10.3969/j.issn.1008-9578.2018.05.019

国家粮食公益性行业科研专项（201313012）；

本校为第一机构

以美藤果油所得到的美藤果粕为原料,通过单因素试验和Box-Behnken响应面法优化,得到响应面法优化超声辅助碱溶酸沉法制备美藤果蛋白的优化工艺条件为p H 9.8、浸提时间1.8 h、浸提温度49℃、液料比10∶1（m L/g）,此时的美藤果蛋白质的提取率为75%。通过糖化酶酶解美藤果蛋白,纯化后的蛋白纯度可达90%。

The raw materials of Plukenetia volubilis Linneo obtained by ultrasonic-assisted leaching were used as raw materials. The ultrasonic-assisted alkali solubilization and the preparation of Plukenetia volubilis Linneo were optimized by single factor test and Box-Behnken response surface method. The optimum conditions were as follows： p H 9.8, extraction time 1.8 h, extraction temperature 49 °C, ratio of liquid to solid 10 ∶ 1（m L/g）, and the extraction rate of the protein was 75%. Plukenetia volubilis Linneo protein was enzymatic hydrolys...