期刊论文

Antivirus effects of polysaccharides from Sargassum fusiforme in vitro

中文

中国病理生理杂志

Chinese Journal of Pathophysiology

1000-4718

2004

20

5

765-768

10.3321/j.issn:1000-4718.2004.05.015

广东省“十五“重大科技专项(2002A3050503)； 广东省自然科学基金研究团队项目(039213)； 广东省重点科研成果推广项目粤财企(2001)382号-C40205； 广东省重点基金项目(013203)；

本校为其他机构

目的:观察羊栖菜多糖及其分离产物对单纯疱疹病毒1型(HSV-1)和柯萨奇病毒(CVB3)所引起细胞病变(CPE)的抑制作用及其机理.方法:采用MTT比色法,测定羊栖菜多糖及其分离产物对Vero细胞的细胞毒性,并在对细胞的最大无毒浓度(MNCC)范围内,采用体外细胞培养法研究羊栖菜多糖样品对上述两种病毒的抑制作用.在此基础上采用分组实验初步探讨了多糖样品的抗病毒机理.结果与结论:①羊栖菜多糖样品对Vero细胞的毒性较小,CC50均大于5 000 mg/L.②除ALG外,其它样品对HSV-1均有明显的抗病毒作用,且随着纯度的提高,样品抗病毒作用随之增强,其中F1、F2和F4抗病毒效果优于ACV.③羊栖菜多糖样品对CVB3的抗病毒效果均...

AIM: To assay antiviral effects of the polysaccharide and separated products from Sargassum fusiforme on herpes simplex virus type 1 (HSV-1) and coxsackie virus (CVB 3) and their antiviral mechanism. METHODS: The antiviral effects of all extracts from Sargassum fusiforme on HSV-1 and CVB 3 and their cytotoxicity on Vero cells were studied by CPE and MTT methods. Antiviral mechanism of two extracts was also investigated by four different ways. RESULTS and CONCLUSION: ① The cytotoxicity of extracts on Vero cells was undetectable (CC 50> 5 000 mg...