To improve the microorganism oil extraction efficiency, microwave was adopted to break bacte- rium cells and the process of methanol extinction grease was researched. By measuring the cellular pro- teins release quantity, the better condition of microwave was 420 W in 2 rain. Scanning electron micros- copy analysis of the broken bacterium showed that there were holes and cracks in hyphae, and the struc- ture was destroyed. So the effect of microwave broken was perfect. Then, by the reaction of methyl direct- ly and orthogonal test, analyzing th...