期刊论文

中文

中国中药杂志

1001-5302

2015

40

11

2084-2089

10.4268/cjcmm20151105

国家自然科学基金项目（81274023）；

本校为第一机构

药用植物珠子参的根茎在中国西南区域已有上千年的药用历史,三萜皂苷为珠子参最主要的活性成分。为了探索珠子参根茎中皂苷物质生物合成的分子基础,该文采用Illumina HiSeq 2000高通量测序获得珠子参根茎的转录组数据;使用 Trinity 和TGICL 软件实现Unigene 的de novo 拼接;基于BLAST 完成Unigene 的蛋白功能注释、KOG 功能注释、GO 分类和 KEGG 代谢通路分析。最终获得了短读序15. 6 Gb,通过de novo 拼接注释得到Unigene 62 240个。研究发现,珠子参根茎部表达的19个Unigene 与三萜碳环骨架合成相关;69个Unigene 与介导三萜碳环骨架的氧化和羟基化等修饰的CYP450相关,18个Unigene 与负责三萜碳环...

The rhizome of Panax japonicus var. major have been used as the natural medicinal agent by Chinese traditional doctors for more than thousand years. Most of the therapeutic effects of P. japonicus var. major had been reported due to the presence of tetracyclic or pentacyclic triterpene saponins. In this study, Illumina pair-end RNA-sequencing and de novo splicing were done in order to understand the pathway of triterpenoid saponins in this species. The valid reads data of 15. 6 Gb were obtained. The 62 240 unigenes were finally obtained by de novo splicing. After annotation, we discovered 19 u...