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Enhancement of docosahexaenoic acid production by low-energy ion implantation coupled with screening method based on Sudan black B staining in Schizochytrium sp.

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成果类型:
期刊论文
作者:
Fu, Jie;Chen, Tao;Lu, Hao;Lin, Yuanfeng;Xie, Xinlei;...
通讯作者:
Zheng, Cao
作者机构:
[He, Dongping; Tian, Hua; Fu, Jie; Lu, Hao; Xie, Xinlei; Lin, Yuanfeng; Zheng, Cao] Wuhan Polytech Univ, Coll Food Sci & Engn, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.
[Chen, Tao] Chinese Acad Sci, Wuhan Inst Virol, 44 Xiaohongshan, Wuhan 430071, Hubei, Peoples R China.
通讯机构:
[Zheng, Cao] W
Wuhan Polytech Univ, Coll Food Sci & Engn, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.
语种:
英文
关键词:
DHA;Lipid;Low-energy ion implantation;Schizochytrium sp.;Sudan black B staining
期刊:
Bioresource Technology
ISSN:
0960-8524
年:
2016
卷:
221
页码:
405-411
基金类别:
Acknowledgements This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. We especially thank Prof. Jing Shu from College of Food Science and Engineering of Wuhan Polytechnic University for her assistance in GC–MS data analysis.
机构署名:
本校为第一且通讯机构
院系归属:
食品科学与工程学院
摘要:
Schizochytrium sp. is a hopeful docosahexaenoic acid (DHA) producing candidate due to its rapid growth rate and high DHA proportion in total lipid content. In this study, low-energy ion implantation was applied to Schizochytrium sp. to induce high DHA-producing mutants. Screening these mutants by Sudan black B staining, a mutant strain S1 which showed a 61% improvement in DHA production than that of the parent strain was successfully selected. Subsequently, parameters of DHA production of mutant strain S1 were optimized in a 500-mL Erlenmeyer f...

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