期刊论文

中文

中国兽医科学

1673-4696

2010

40

1

103-106

10.16656/j.issn.1673-4696.2010.01.005

本校为第一机构

采用甲醇-100 g/L三氯乙酸混合液(二者体积比为50:50)提取血浆中的硫酸黏杆菌素,用邻苯二甲醛进行柱前衍生.荧光检测,激发波长344 nm,发射波长436 nm.流动相为V_(乙腈):V_(0.01 mol/L磷酸缓冲液)=68:32,流速为1.4 mL/min.猪、鸡血浆中硫酸黏杆菌素浓度为0.1～4 μg/mL时,硫酸黏杆菌素与其峰面积之间存在良好的线性关系(r~2=0.997),最低定量限为0.1 μg/mL.血浆中添加0.1、0.5、2.0μg/mL浓度的硫酸黏杆菌素,其回收率>70%,变异系数均

Colistin sulfate was extracted from plasma of swine and chicken with 100 g/L trichloroacetic acid-methanol（50 ： 50,V/V） and the extracted colistin sulfate was derived with OPA before detection. The samples were injected into the detector automatically and detected by high performance liquid chromato-graphic（HPLC） with fluorescence detector at an excitation wavelength of 344 nm and an emission wave-length of 436 nm. The mobile phase consisted of acetonitrile-0. 01 mol/L potassium phosphate buffer （68 ： 32,V/V） and the flow rate was 1.4 m...