期刊论文

中文

中国兽医科学

1673-4696

2024

54

05

696-701

10.16656/j.issn.1673-4696.2024.0079

U22A20514,2022:国家自然科学基金 202303:武汉轻工大学动物营养与饲料科学湖北省重点实验室开放课题

本校为第一机构

不同浓度葛根素(PR)、丙酮酸乙酯(EP)、酪醇(TS)、氧化锌(ZnO)处理IPEC-1 细胞,CCK-8 法检测细胞活力;乳酸脱氢酶(lactate dehydrogenase,LDH)试剂盒检测几种功能性物质对ETEC K88 感染IPEC-1细胞损伤程度的影响;实时荧光定量PCR法检测不同功能性物质对ETEC K88 感染IPEC-1 细胞相关基因相对表达水平的影响.结果:PR、EP和ZnO均可促进IPEC-1 细胞增殖,其最适添加浓度分别为 5 μmol/L、1 mmol/L和 1 μmol/L;TS无促进IPEC-1 细胞增殖作用.ETEC K88 感染IPEC-1 细胞后导致LDH活性显著升高(P＜0.05);与K88 组相比,添加 5 μmol/L PR、1 mmol/L EP和 1 μmol/L ZnO均能显著降低LDH活性(P＜0.05).ETE...

IPEC-1 cells were treated with different concentrations of puerarin(PR),ethyl pyruvate(EP),tyrosol(TS)or zinc oxide(ZnO),and then cell viability was detected by CCK-8 method.The lactate dehydrogenase(LDH)kit was used to detect the effect of functional substances on the cytotoxic effects of ETEC K88.Real-time PCR was used to detect the effects of different functional substances on the rel-ative expression levels of genes related to IPEC-1 cells infected with ETEC K88.Results ∶PR,EP and ZnO could promote the proliferation of IPEC-1 cells,and the...